解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::For enteroviruses such as poliovirus (PV), empty capsids, which are antigenically indistinguishable from mature virions, are produced naturally during viral infection. The production of such capsids recombinantly, in heterologous systems such as yeast, have great potential as virus-like particle (VLP) vaccine candidat...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00838-19
更新日期:2020-03-11 00:00:00
abstract::The wide distribution of colistin-resistant bacteria in developing countries has become a common phenomenon. To understand the mechanisms underlying their distribution, we studied the mcr genetic background of colistin-resistant Escherichia coli isolates from the fecal microbiota of healthy human residents from a comm...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00117-20
更新日期:2020-03-04 00:00:00
abstract::Neal Hammer works in the field of bacterial pathogenesis, metabolism, and antibiotic resistance. In this mSphere of Influence article, he reflects on how "Gut inflammation provides a respiratory electron acceptor for Salmonella" by Winter and colleagues (S. E. Winter, P. Thiennimitr, M. G. Winter, B. P. Butler, et al....
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00110-20
更新日期:2020-02-26 00:00:00
abstract::Toxoplasma gondii is a ubiquitous, intracellular protozoan that extensively modifies infected host cells through secreted effector proteins. Many such effectors must be translocated across the parasitophorous vacuole (PV), in which the parasites replicate, ultimately ending up in the host cytosol or nucleus. This tran...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00858-19
更新日期:2020-02-19 00:00:00
abstract::Microsporidia are obligate intracellular parasites that infect a wide variety of host organisms, including humans. The sporoplasm is the initial stage of microsporidian infection and proliferation, but its morphological and molecular characteristics are poorly understood. In this study, the sporoplasm of Nosema bombyc...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00958-19
更新日期:2020-02-12 00:00:00
abstract::Parasitic protozoans of the genus Theileria are intracellular pathogens that induce the cellular transformation of leukocytes, causing uncontrolled proliferation of the infected host cell. The transforming stage of the parasite has a strictly intracellular lifestyle and ensures its distribution to both daughter cells ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00709-19
更新日期:2020-02-05 00:00:00
abstract::Toxoplasma gondii can infect and replicate in vascular endothelial cells prior to entering host tissues. However, little is known about the molecular interactions at the parasite-endothelial cell interface. We demonstrate that T. gondii infection of primary human umbilical vein endothelial cells (HUVEC) altered cell m...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00550-19
更新日期:2020-01-29 00:00:00
abstract::Candida auris, an emerging multidrug-resistant yeast, has recently been associated with outbreaks of invasive infections in health care facilities worldwide. Its success as a nosocomial pathogen lies in its capability to sustain for prolonged periods in the intensive care unit (ICU), adeptly colonize skin, and spread ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00972-19
更新日期:2020-01-22 00:00:00
abstract::Diana V. Pastrana works in the field of DNA tumor virus biology. In this mSphere of Influence article, she reflects on how the two papers "Donor origin of BKV replication after kidney transplantation" (C. Schmitt, L. Raggub, S. Linnenweber-Held, O. Adams, et al., J Clin Virol 59:120-125, 2014, https://doi.org/10.1016/...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00964-19
更新日期:2020-01-15 00:00:00
abstract::Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide and is associated with high rates of mortality and growth stunting in children inhabiting low- to middle-resource countries. To better understand the impact of breastfeeding on Campylobacter infection in infants in sub-Saharan Africa and South Asi...
journal_title:mSphere
pub_type: 杂志文章,多中心研究
doi:10.1128/mSphere.00735-19
更新日期:2020-01-15 00:00:00
abstract::Hypervirulent Klebsiella pneumoniae (hvKp) is an emerging pathogen of increasing concern due to its ability to cause serious organ and life-threatening infections in healthy individuals and its increasing acquisition of antimicrobial resistance determinants. Identification of hvKp is critical for patient care and epid...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00850-19
更新日期:2020-01-08 00:00:00
abstract::Prebiotic oligosaccharides, such as fructooligosaccharides, are increasingly being used to modulate the composition and activity of the gut microbiota. However, carbohydrate utilization analyses and metagenomic studies recently revealed the ability of deleterious and uncultured human gut bacterial species to metaboliz...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00771-19
更新日期:2020-01-08 00:00:00
abstract::Molecular detection of biological agents in the field has traditionally relied on the use of quantitative real-time PCR (qPCR), which now includes commercially available instruments that can be used in the laboratory or field. Adapting this technology for field-forward applications necessitated innovation to minimize ...
journal_title:mSphere
pub_type: 杂志文章,评审
doi:10.1128/mSphere.00695-19
更新日期:2019-12-11 00:00:00
abstract::Vibrio cholerae is a noninvasive pathogen that colonizes the small intestine and produces cholera toxin, causing severe secretory diarrhea. Cholera results in long lasting immunity, and recent studies have improved our understanding of the antigenic repertoire of V. cholerae Interactions between the host, V. cholerae,...
journal_title:mSphere
pub_type: 杂志文章,评审
doi:10.1128/mSphere.00597-19
更新日期:2019-11-27 00:00:00
abstract::Huanglongbing disease (HLB; yellow shoot disease) is a severe worldwide infectious disease for citrus family plants. The pathogen "Candidatus Liberibacter asiaticus" is an alphaproteobacterium of the Rhizobiaceae family that has been identified as the causative agent of HLB. The virulence of "Ca. Liberibacter asiaticu...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00409-19
更新日期:2019-11-13 00:00:00
abstract::To design immune interventions that can synergize with antiretroviral therapy (ART) to reduce the rate of HIV mother-to-child transmission (MTCT), it is essential to characterize maternal immune responses in the setting of ART during pregnancy and breastfeeding and define their effect on MTCT. Prior studies reported a...
journal_title:mSphere
pub_type: 杂志文章,随机对照试验
doi:10.1128/mSphere.00716-19
更新日期:2019-10-23 00:00:00
abstract::Little is known regarding how the chronic Toxoplasma gondii cyst develops. Here, we investigated intravacuolar-network-associated dense granule (GRA) proteins GRA1, GRA2, GRA4, GRA6, GRA9, and GRA12 during cyst development in vitro after differentiation of the tachyzoite-stage parasitophorous vacuole. By day 1 postdif...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00487-19
更新日期:2019-10-16 00:00:00
abstract::Cryptococcus gattii R265 is a hypervirulent fungal strain responsible for the recent outbreak of cryptococcosis in Vancouver Island of British Columbia in Canada. It differs significantly from Cryptococcus neoformans in its natural environment, its preferred site in the mammalian host, and its pathogenesis. Our previo...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00644-19
更新日期:2019-10-09 00:00:00
abstract::Felipe Santiago-Tirado studies the cell biology of cryptococcal infections. In this mSphere of Influence article, he reflects on how the papers "Systematic Genetic Analysis of Virulence in the Human Fungal Pathogen Cryptococcus neoformans" (https://doi.org/10.1016/j.cell.2008.07.046) and "Unraveling the Biology of a F...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00647-19
更新日期:2019-10-02 00:00:00
abstract::Lassa virus (LASV), a member of the family Arenaviridae, is the causative agent of Lassa fever. Lassa virus is endemic in West African countries, such as Nigeria, Guinea, Liberia, and Sierra Leone, and causes outbreaks annually. Lassa fever onset begins with "flu-like" symptoms and may develop into lethal hemorrhagic ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00428-19
更新日期:2019-09-25 00:00:00
abstract::Michael D. L. Johnson is a molecular microbiologist observing the role of metals in bacterial biology. In this mSphere of Influence article, he discusses the impact that the paper "Decoupling of the minority PhD talent pool and assistant professor hiring in medical school basic science departments in the US" by Kennet...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00599-19
更新日期:2019-09-18 00:00:00
abstract::Antibiotic resistance is a global crisis that threatens our ability to treat bacterial infections, such as tuberculosis, caused by Mycobacterium tuberculosis Of the 10 million cases of tuberculosis in 2017, approximately 19% of new cases and 43% of previously treated cases were caused by strains of M. tuberculosis res...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00606-19
更新日期:2019-09-11 00:00:00
abstract::The genomes of Leishmania and trypanosomes encode six paralogs of the eIF4E cap-binding protein, known in other eukaryotes to anchor the translation initiation complex. In line with the heteroxenous nature of these parasites, the different LeishIF4E paralogs vary in their biophysical features and their biological beha...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00450-19
更新日期:2019-09-04 00:00:00
abstract::Jorge Amich studies several aspects of sulfur and nitrogen metabolism in Aspergillus fumigatus, with the ultimate aim of identifying targets for the development of novel antifungals. In this mSphere of Influence article, he reflects on how "Sub-Telomere Directed Gene Expression during Initiation of Invasive Aspergillo...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00566-19
更新日期:2019-08-21 00:00:00
abstract::The genetic stability of every living organism depends on accurate DNA replication and repair systems. Here, we investigated the Aspergillus fumigatusMSH2 mismatch repair (MMR) gene MshA and how it impacts virulence and the evolution of azole resistance. We examined mshA gene variation in 62 environmental and clinical...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00416-19
更新日期:2019-08-07 00:00:00
abstract::The fungal cell wall is a complex and dynamic entity essential for the development of fungi. It is composed mainly of polysaccharides that are synthetized by protein complexes. At the cell wall level, enzyme activities are involved in postsynthesis polysaccharide modifications such as cleavage, elongation, branching, ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00397-19
更新日期:2019-07-31 00:00:00
abstract::The role of the gut microbiome in critical illness is being actively investigated, but the optimal sampling methods for sequencing studies of gut microbiota remain unknown. Stool samples are generally considered the reference standard but are not practical to obtain in the intensive care unit (ICU), and thus, rectal s...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00358-19
更新日期:2019-07-24 00:00:00
abstract::Commensal and generally harmless in healthy individuals, Enterococcus faecalis causes opportunistic infections in immunocompromised patients. Plasmid-cured E. faecalis strain VE14089, derived from sequenced reference strain V583, is widely used for functional studies due to its improved genetic amenability. Although s...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00310-19
更新日期:2019-07-10 00:00:00
abstract::Megan E. Spurgeon works in the field of viral oncology. In this mSphere of Influence article, she reflects on how the paper "Rescuing US biomedical research from its systemic flaws" by Bruce Alberts, Marc W. Kirschner, Shirley Tilghman, and Harold Varmus (Proc Natl Acad Sci U S A 111:5773-5777, 2014, https://doi.org/1...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00318-19
更新日期:2019-07-03 00:00:00
abstract::Brucella, the agent causing brucellosis, is a major zoonotic pathogen with worldwide distribution. Brucella resides and replicates inside infected host cells in membrane-bound compartments called Brucella-containing vacuoles (BCVs). Following uptake, Brucella resides in endosomal BCVs (eBCVs) that gradually mature fro...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00380-19
更新日期:2019-06-26 00:00:00
abstract::J. Christian Pérez studies the interplay between Candida albicans and the mammalian host. In this mSphere of Influence article, he reflects on how "Systematic screens of a Candida albicans homozygous deletion library decouple morphogenetic switching and pathogenicity" (S. M. Noble, S. French, L. A. Kohn, V. Chen, et a...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00258-19
更新日期:2019-06-19 00:00:00
abstract::The azithromycin resistance conferred by phosphotransferase is encoded in the gene mph(A). This gene has been discovered in and reported for many bacterial species. We examined the prevalence of azithromycin resistance in Vibrio fluvialis (AR-VF) isolated during 2014 to 2015 from the hospitalized acute diarrheal patie...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00215-19
更新日期:2019-06-12 00:00:00
abstract::Methane-oxidizing microorganisms perform an important role in reducing emissions of the greenhouse gas methane to the atmosphere. To date, known bacterial methanotrophs belong to the Proteobacteria, Verrucomicrobia, and NC10 phyla. Within the Proteobacteria phylum, they can be divided into type Ia, type Ib, and type I...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00631-18
更新日期:2019-06-05 00:00:00
abstract::Newborns are constantly exposed to various microbes from birth; hence, diverse commensal bacteria colonize the oral cavity. However, how or when these bacteria construct a complex and stable ecosystem remains unclear. This prospective cohort study examined the temporal changes in bacterial diversity and composition in...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00187-19
更新日期:2019-05-29 00:00:00
abstract::Salmonella genomic island 1 (SGI1) is an integrative genetic island first described in Salmonella enterica serovars Typhimurium DT104 and Agona in 2000. Variants of it have since been described in multiple serovars of S. enterica, as well as in Proteus mirabilis, Acinetobacter baumannii, Morganella morganii, and sever...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00169-19
更新日期:2019-05-22 00:00:00
abstract::The fungal pathogen Candida glabrata can cause both mucosal and disseminated infections. Cell adhesion, a key step in colonization and infection, depends in C. glabrata primarily on the Epa family of cell adhesion proteins. While Epa proteins have been documented to mediate specific adhesion to host glycans, some of t...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00277-19
更新日期:2019-05-01 00:00:00
abstract::Bordetella pertussis, B. parapertussis, and B. bronchiseptica cause respiratory infections, many of which are characterized by coughing of the infected hosts. The pathogenesis of the coughing remains to be analyzed, mainly because there were no convenient infection models of small animals that replicate coughing after...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00093-19
更新日期:2019-04-24 00:00:00
abstract::Elizabethkingia anophelis is an emerging global multidrug-resistant opportunistic pathogen. We assessed the diversity among 13 complete genomes and 23 draft genomes of E. anophelis strains derived from various environmental settings and human infections from different geographic regions around the world from 1950s to ...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00040-19
更新日期:2019-04-03 00:00:00
abstract::Although the role of high-risk human papillomaviruses (hrHPVs) as etiological agents in cancer development has been intensively studied during the last decades, there is still the necessity of understanding the impact of the HPV E6 and E7 oncogenes on host cells, ultimately leading to malignant transformation. Here, w...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00129-19
更新日期:2019-03-27 00:00:00
abstract::Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt to improve the protocols used for EV isolation, we explored a model of vesicle production after growth of Cryptococcus gattii and Cryptococcus neoformans on solid medi...
journal_title:mSphere
pub_type: 杂志文章
doi:10.1128/mSphere.00080-19
更新日期:2019-03-20 00:00:00